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Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis

The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology chassis, such as Escherichia coli and Bacillus subtilis, constitutes one of the major hurdles of the rational genome engineering. Using lambda Red recombineering we integrated the thermosensitive lamb...

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Detalles Bibliográficos
Autores principales: Juhas, Mario, Wong, Christine, Ajioka, James W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5087902/
https://www.ncbi.nlm.nih.gov/pubmed/27798678
http://dx.doi.org/10.1371/journal.pone.0165778