Enzyme-catalyzed expressed protein ligation

Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-Cys containing peptides but the requirement of a Cys residue at the ligation junction can limit its use. Here we employ subtiligase variants to eff...

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Detalles Bibliográficos
Autores principales: Henager, Samuel H., Chu, Nam, Chen, Zan, Bolduc, David, Dempsey, Daniel R., Hwang, Yousang, Wells, James, Cole, Philip A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088058/
https://www.ncbi.nlm.nih.gov/pubmed/27669326
http://dx.doi.org/10.1038/nmeth.4004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088058/
https://www.ncbi.nlm.nih.gov/pubmed/27669326
http://dx.doi.org/10.1038/nmeth.4004

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