In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI

Atmospheric pressure MALDI on a Q-Exactive instrument was optimized for in-source decay and pseudo-MS(3). The dependence of AP-MALDI ISD on the MALDI liquid matrix was investigated for peptides and proteins. The liquid matrices enabled long-life ISD signal, and exhibited high fragment ion yield and...

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Autores principales: Ait-Belkacem, Rima, Dilillo, Marialaura, Pellegrini, Davide, Yadav, Avinash, de Graaf, Erik L., McDonnell, Liam A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Application Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088222/
https://www.ncbi.nlm.nih.gov/pubmed/27752913
http://dx.doi.org/10.1007/s13361-016-1511-0
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author Ait-Belkacem, Rima
Dilillo, Marialaura
Pellegrini, Davide
Yadav, Avinash
de Graaf, Erik L.
McDonnell, Liam A.
author_facet Ait-Belkacem, Rima
Dilillo, Marialaura
Pellegrini, Davide
Yadav, Avinash
de Graaf, Erik L.
McDonnell, Liam A.
author_sort Ait-Belkacem, Rima
collection PubMed
description Atmospheric pressure MALDI on a Q-Exactive instrument was optimized for in-source decay and pseudo-MS(3). The dependence of AP-MALDI ISD on the MALDI liquid matrix was investigated for peptides and proteins. The liquid matrices enabled long-life ISD signal, and exhibited high fragment ion yield and signal stability. Extensive a-, b-, c-, y-, and z-type fragment series were observed depending on the matrix used but were most extensive with 2,5-DHB. Complete sequence coverage of small peptide and intact protein-terminus sequence tags were obtained and confirmed using HCD as a pseudo-MS(3) method. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13361-016-1511-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-50882222016-11-16 In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI Ait-Belkacem, Rima Dilillo, Marialaura Pellegrini, Davide Yadav, Avinash de Graaf, Erik L. McDonnell, Liam A. J Am Soc Mass Spectrom Application Note Atmospheric pressure MALDI on a Q-Exactive instrument was optimized for in-source decay and pseudo-MS(3). The dependence of AP-MALDI ISD on the MALDI liquid matrix was investigated for peptides and proteins. The liquid matrices enabled long-life ISD signal, and exhibited high fragment ion yield and signal stability. Extensive a-, b-, c-, y-, and z-type fragment series were observed depending on the matrix used but were most extensive with 2,5-DHB. Complete sequence coverage of small peptide and intact protein-terminus sequence tags were obtained and confirmed using HCD as a pseudo-MS(3) method. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13361-016-1511-0) contains supplementary material, which is available to authorized users. Springer US 2016-10-17 2016 /pmc/articles/PMC5088222/ /pubmed/27752913 http://dx.doi.org/10.1007/s13361-016-1511-0 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Application Note
Ait-Belkacem, Rima
Dilillo, Marialaura
Pellegrini, Davide
Yadav, Avinash
de Graaf, Erik L.
McDonnell, Liam A.
In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title_full In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title_fullStr In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title_full_unstemmed In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title_short In-Source Decay and Pseudo-MS(3) of Peptide and Protein Ions Using Liquid AP-MALDI
title_sort in-source decay and pseudo-ms(3) of peptide and protein ions using liquid ap-maldi
topic Application Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5088222/
https://www.ncbi.nlm.nih.gov/pubmed/27752913
http://dx.doi.org/10.1007/s13361-016-1511-0
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