Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates

We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl‐tRNA synthetase/tRNA(CUA) pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal...

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Detalles Bibliográficos
Autores principales: Stanley, Mathew, Virdee, Satpal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094518/
https://www.ncbi.nlm.nih.gov/pubmed/27197715
http://dx.doi.org/10.1002/cbic.201600138

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094518/
https://www.ncbi.nlm.nih.gov/pubmed/27197715
http://dx.doi.org/10.1002/cbic.201600138

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