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Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates
We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl‐tRNA synthetase/tRNA(CUA) pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal...
Autores principales: | Stanley, Mathew, Virdee, Satpal |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094518/ https://www.ncbi.nlm.nih.gov/pubmed/27197715 http://dx.doi.org/10.1002/cbic.201600138 |
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