EasyClone‐MarkerFree: A vector toolkit for marker‐less integration of genes into Saccharomyces cerevisiae via CRISPR‐Cas9

Saccharomyces cerevisiae is an established industrial host for production of recombinant proteins, fuels and chemicals. To enable stable integration of multiple marker‐free overexpression cassettes in the genome of S. cerevisiae, we have developed a vector toolkit EasyClone‐MarkerFree. The integrati...

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Detalles Bibliográficos
Autores principales: Jessop‐Fabre, Mathew M, Jakočiūnas, Tadas, Stovicek, Vratislav, Dai, Zongjie, Jensen, Michael K, Keasling, Jay D, Borodina, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEYߚVCH Verlag 2016
Materias:
Biotech Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094547/
https://www.ncbi.nlm.nih.gov/pubmed/27166612
http://dx.doi.org/10.1002/biot.201600147

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094547/
https://www.ncbi.nlm.nih.gov/pubmed/27166612
http://dx.doi.org/10.1002/biot.201600147

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