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Environmental Topology and Water Availability Modulates the Catalytic Activity of β-Galactosidase Entrapped in a Nanosporous Silicate Matrix

In the present work we studied the catalytic activity of E. coli β-Gal confined in a nanoporous silicate matrix (E(β-Gal)) at different times after the beginning of the sol-gel polymerization process. Enzyme kinetic experiments with two substrates (ONPG and PNPG) that differed in the rate-limiting s...

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Detalles Bibliográficos
Autores principales: Burgos, M. Ines, Velasco, Manuel I., Acosta, Rodolfo H., Perillo, María A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095660/
https://www.ncbi.nlm.nih.gov/pubmed/27811995
http://dx.doi.org/10.1038/srep36593