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Environmental Topology and Water Availability Modulates the Catalytic Activity of β-Galactosidase Entrapped in a Nanosporous Silicate Matrix
In the present work we studied the catalytic activity of E. coli β-Gal confined in a nanoporous silicate matrix (E(β-Gal)) at different times after the beginning of the sol-gel polymerization process. Enzyme kinetic experiments with two substrates (ONPG and PNPG) that differed in the rate-limiting s...
Autores principales: | Burgos, M. Ines, Velasco, Manuel I., Acosta, Rodolfo H., Perillo, María A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095660/ https://www.ncbi.nlm.nih.gov/pubmed/27811995 http://dx.doi.org/10.1038/srep36593 |
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