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Tissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies

Analysis of the tissue distribution of plasmid DNA after administration of nonviral gene delivery systems is best accomplished using quantitative real-time polymerase chain reaction (qPCR), although published strategies do not allow determination of the absolute mass of plasmid delivered to differen...

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Detalles Bibliográficos
Autores principales: Ho, Joan K, White, Paul J, Pouton, Colin W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095683/
https://www.ncbi.nlm.nih.gov/pubmed/27701400
http://dx.doi.org/10.1038/mtna.2016.79