Cargando…

Tissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies

Analysis of the tissue distribution of plasmid DNA after administration of nonviral gene delivery systems is best accomplished using quantitative real-time polymerase chain reaction (qPCR), although published strategies do not allow determination of the absolute mass of plasmid delivered to differen...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ho, Joan K, White, Paul J, Pouton, Colin W
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095683/ https://www.ncbi.nlm.nih.gov/pubmed/27701400 http://dx.doi.org/10.1038/mtna.2016.79

Ejemplares similares

A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
por: Sivaganesan, Mano, et al.
Publicado: (2008)

Absolute quantification by droplet digital PCR versus analog real-time PCR
por: Hindson, Christopher M, et al.
Publicado: (2013)

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay
por: Lin, Chih-Hui, et al.
Publicado: (2011)

Digital PCR provides sensitive and absolute calibration for high throughput sequencing
por: White, Richard A, et al.
Publicado: (2009)

Correction: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
por: White, Richard A, et al.
Publicado: (2009)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants
por: Stopfer, Lauren E., et al.
Publicado: (2021)

FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
por: Verderio, Paolo, et al.
Publicado: (2008)

Multiplex qPCR for Detection and Absolute Quantification of Malaria
por: Kamau, Edwin, et al.
Publicado: (2013)

Biodistribution and blood clearance of plasmid DNA administered in arginine peptide complexes
por: Woo, Jung Gyu, et al.
Publicado: (2011)

Quantitative Evaluation of CFTR Gene Expression: A Comparison between Relative Quantification by Real-Time PCR and Absolute Quantification by Droplet Digital PCR
por: Bruno, Sabina Maria, et al.
Publicado: (2023)

Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
por: Shirima, Rudolph R., et al.
Publicado: (2017)

Self-Crosslinking Lipopeptide/DNA/PEGylated Particles: A New Platform for DNA Vaccination Designed for Assembly in Aqueous Solution
por: Ho, Joan K., et al.
Publicado: (2018)

Optimized Quantification of Fragmented, Free Circulating DNA in Human Blood Plasma Using a Calibrated Duplex Real-Time PCR
por: Horlitz, Martin, et al.
Publicado: (2009)

A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification
por: Rutledge, Robert G.
Publicado: (2011)

A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
por: Mournetas, Virginie, et al.
Publicado: (2016)

Impact of variance components on reliability of absolute quantification using digital PCR
por: Jacobs, Bart KM, et al.
Publicado: (2014)

Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
por: Pavšič, Jernej, et al.
Publicado: (2015)

Corrigendum: A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR
por: Mournetas, Virginie, et al.
Publicado: (2019)

Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach
por: Nuraeni, Umi, et al.
Publicado: (2023)

Investigation of absolute dose calibration accuracy for TomoTherapy using real water
por: Pang, Tingtian, et al.
Publicado: (2021)

Absolute quantification of SARS-CoV-2 with Clarity Plus™ digital PCR
por: Tan, Shawn Yi Han, et al.
Publicado: (2022)

Absolute quantification of somatic DNA alterations in human cancer
por: Carter, Scott L., et al.
Publicado: (2012)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR
por: Plotka, Magdalena, et al.
Publicado: (2017)

CMS preshower in-situ absolute calibration
por: Evangelou, I
Publicado: (2006)

Absolute momentum calibration of the HARP TPC
por: Catanesi, M G, et al.
Publicado: (2008)

A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR
por: White, H, et al.
Publicado: (2015)

Droplet Digital PCR for absolute quantification and determination of proviral load of HTLV-1 and 2
por: Hedberg, Sara Thulin, et al.
Publicado: (2015)

Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR
por: D’Alessandra, Yuri, et al.
Publicado: (2022)

Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material
por: Dong, Lianhua, et al.
Publicado: (2015)

Measurement of altered APP isoform expression in adipose tissue of diet-induced obese mice by absolute quantitative real-time PCR
por: Min, Hansol, et al.
Publicado: (2017)

A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR
por: He, Kuang, et al.
Publicado: (2019)

Quantification of Mycobacterium avium subspecies in pig tissues by real-time quantitative PCR
por: Tirkkonen, Taneli, et al.
Publicado: (2013)

Problems of Calibration of Absolute Magnitudes and Temperature of Stars
por: Hauck, B, et al.
Publicado: (1973)

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR
por: Busby, Eloise, et al.
Publicado: (2017)

Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification
por: Tao, Hui, et al.
Publicado: (2016)

CMV DNA quantification in plasma using an automated real-time PCR assay
por: Yerly, S., et al.
Publicado: (2006)

A novel procedure for absolute real-time quantification of gene expression patterns
por: Lu, Yingqing, et al.
Publicado: (2012)

Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs
por: Miura, Fumihito, et al.
Publicado: (2008)

Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples
por: Rocchigiani, Angela M., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_6ie7lsnfoc92utpo85bbmbafno