Conjugation and Evaluation of Triazole‐Linked Single Guide RNA for CRISPR‐Cas9 Gene Editing

The CRISPR‐Cas9 gene editing system requires Cas9 endonuclease and guide RNAs (either the natural dual RNA consisting of crRNA and tracrRNA or a chimeric single guide RNA) that direct site‐specific double‐stranded DNA cleavage. This communication describes a click ligation approach that uses alkyne–...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Kaizhang, Chou, Eldon T., Begay, Shawn, Anderson, Emily M., van Brabant Smith, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096024/
https://www.ncbi.nlm.nih.gov/pubmed/27441384
http://dx.doi.org/10.1002/cbic.201600320

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096024/
https://www.ncbi.nlm.nih.gov/pubmed/27441384
http://dx.doi.org/10.1002/cbic.201600320

Ejemplares similares