Assessing the impact of minimizing arginine conversion in fully defined SILAC culture medium in human embryonic stem cells

We present a fully defined culture system (adapted Essential8(TM) [E8(TM)] medium in combination with vitronectin) for human embryonic stem cells that can be used for SILAC purposes. Although a complete incorporation of the labels was observed after 4 days in culture, over 90% of precursors showed a...

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Detalles Bibliográficos
Autores principales: Scheerlinck, Ellen, Van Steendam, Katleen, Daled, Simon, Govaert, Elisabeth, Vossaert, Liesbeth, Meert, Paulien, Van Nieuwerburgh, Filip, Van Soom, Ann, Peelman, Luc, De Sutter, Petra, Heindryckx, Björn, Dhaenens, Maarten, Deforce, Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096064/
https://www.ncbi.nlm.nih.gov/pubmed/27392809
http://dx.doi.org/10.1002/pmic.201600174

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096064/
https://www.ncbi.nlm.nih.gov/pubmed/27392809
http://dx.doi.org/10.1002/pmic.201600174

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