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A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen

In this study, we present a quadruple immunostaining method for rapid muscle fiber typing of mice and rats using antibodies specific to the adult myosin heavy chain (MyHC) isoforms MyHC1, 2A, 2X, and 2B, which are common marker proteins of distinct muscle fiber types. We developed rat monoclonal ant...

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Detalles Bibliográficos
Autores principales: Sawano, Shoko, Komiya, Yusuke, Ichitsubo, Riho, Ohkawa, Yasuyuki, Nakamura, Mako, Tatsumi, Ryuichi, Ikeuchi, Yoshihide, Mizunoya, Wataru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096669/
https://www.ncbi.nlm.nih.gov/pubmed/27814384
http://dx.doi.org/10.1371/journal.pone.0166080