Cargando…
A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis
Affinity panning of large libraries is a powerful tool to identify protein binders. However, panning rounds are followed by the tedious re-screening of the clones obtained to evaluate binders precisely. In a first application of Bead Surface Display (BeSD) we show successful in vitro affinity select...
Autores principales: | Mankowska, Sylwia A., Gatti-Lafranconi, Pietro, Chodorge, Matthieu, Sridharan, Sudharsan, Minter, Ralph R., Hollfelder, Florian |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098251/ https://www.ncbi.nlm.nih.gov/pubmed/27819305 http://dx.doi.org/10.1038/srep36391 |
Ejemplares similares
-
In vitro affinity screening of protein and peptide binders by megavalent bead surface display
por: Diamante, Letizia, et al.
Publicado: (2013) -
Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease
por: Shafee, Thomas, et al.
Publicado: (2015) -
Multiplexed Affinity Characterization of Protein Binders Directly from a Crude
Cell Lysate by Covalent Capture on Suspension Bead Arrays
por: Huovinen, Tuomas, et al.
Publicado: (2021) -
Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process
por: Houlihan, Gillian, et al.
Publicado: (2015) -
“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry
por: Lindenburg, Laurens, et al.
Publicado: (2021)