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The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation
The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although primary cilia form in the quiescent phase, ciliary disassembly occurs when quiescent cells re-enter the proliferative phase. It was shown that...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099117/ https://www.ncbi.nlm.nih.gov/pubmed/25660017 http://dx.doi.org/10.1016/j.celrep.2015.01.003 |
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author | Miyamoto, Tatsuo Hosoba, Kosuke Ochiai, Hiroshi Royba, Ekaterina Izumi, Hideki Sakuma, Tetsushi Yamamoto, Takashi Dynlacht, Brian David Matsuura, Shinya |
author_facet | Miyamoto, Tatsuo Hosoba, Kosuke Ochiai, Hiroshi Royba, Ekaterina Izumi, Hideki Sakuma, Tetsushi Yamamoto, Takashi Dynlacht, Brian David Matsuura, Shinya |
author_sort | Miyamoto, Tatsuo |
collection | PubMed |
description | The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although primary cilia form in the quiescent phase, ciliary disassembly occurs when quiescent cells re-enter the proliferative phase. It was shown that a mitotic kinase, Polo-like kinase 1 (PLK1), is required for cell-proliferation-coupled primary cilia disassembly. Here, we report that kinesin superfamily protein 2A (KIF2A), phosphorylated at T554 by PLK1, exhibits microtubule-depolymerizing activity at the mother centriole to disassemble the primary cilium in a growth-signal-dependent manner. KIF2A-deficient hTERT-RPE1 cells showed the impairment of primary cilia disassembly following growth stimulation. It was also found that the PLK1-KIF2A pathway is constitutively active in cells from patients with premature chromatid separation (PCS) syndrome and is responsible for defective ciliogenesis in this syndrome. These findings provide insights into the roles of the PLK1-KIF2A pathway in physiological cilia disassembly and cilia-associated disorders. |
format | Online Article Text |
id | pubmed-5099117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-50991172016-11-07 The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation Miyamoto, Tatsuo Hosoba, Kosuke Ochiai, Hiroshi Royba, Ekaterina Izumi, Hideki Sakuma, Tetsushi Yamamoto, Takashi Dynlacht, Brian David Matsuura, Shinya Cell Rep Article The primary cilium is an antenna-like, microtubule-based organelle on the surface of most vertebrate cells for receiving extracellular information. Although primary cilia form in the quiescent phase, ciliary disassembly occurs when quiescent cells re-enter the proliferative phase. It was shown that a mitotic kinase, Polo-like kinase 1 (PLK1), is required for cell-proliferation-coupled primary cilia disassembly. Here, we report that kinesin superfamily protein 2A (KIF2A), phosphorylated at T554 by PLK1, exhibits microtubule-depolymerizing activity at the mother centriole to disassemble the primary cilium in a growth-signal-dependent manner. KIF2A-deficient hTERT-RPE1 cells showed the impairment of primary cilia disassembly following growth stimulation. It was also found that the PLK1-KIF2A pathway is constitutively active in cells from patients with premature chromatid separation (PCS) syndrome and is responsible for defective ciliogenesis in this syndrome. These findings provide insights into the roles of the PLK1-KIF2A pathway in physiological cilia disassembly and cilia-associated disorders. 2015-02-05 2015-02-10 /pmc/articles/PMC5099117/ /pubmed/25660017 http://dx.doi.org/10.1016/j.celrep.2015.01.003 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Miyamoto, Tatsuo Hosoba, Kosuke Ochiai, Hiroshi Royba, Ekaterina Izumi, Hideki Sakuma, Tetsushi Yamamoto, Takashi Dynlacht, Brian David Matsuura, Shinya The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title | The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title_full | The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title_fullStr | The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title_full_unstemmed | The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title_short | The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation |
title_sort | microtubule-depolymerizing activity of a mitotic kinesin protein kif2a drives primary cilia disassembly coupled with cell proliferation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099117/ https://www.ncbi.nlm.nih.gov/pubmed/25660017 http://dx.doi.org/10.1016/j.celrep.2015.01.003 |
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