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Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios

The use of tissue homogenate has greatly aided the study of the functioning of mitochondria. However, the amount of ATP produced per oxygen molecule consumed, that is, the effective P/O ratio, has never been measured directly in tissue homogenate. Here we combine and refine existing methods previous...

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Detalles Bibliográficos
Autores principales: Salin, Karine, Villasevil, Eugenia M., Auer, Sonya K., Anderson, Graeme J., Selman, Colin, Metcalfe, Neil B., Chinopoulos, Christos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099967/
https://www.ncbi.nlm.nih.gov/pubmed/27798358
http://dx.doi.org/10.14814/phy2.13007