Serum availability affects expression of common house-keeping genes in colon adenocarcinoma cell lines: implications for quantitative real-time PCR studies

Careful selection of housekeeping genes (HKG) is prerequisite to yield sound qPCR results. HKG expression varies in response to hypoxia but the effect of manipulations of serum availability, a common experimental procedure, remains unknown. Also, no data on HKG expression stability across colon aden...

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Detalles Bibliográficos
Autores principales: Krzystek-Korpacka, Malgorzata, Hotowy, Katarzyna, Czapinska, Elzbieta, Podkowik, Magdalena, Bania, Jacek, Gamian, Andrzej, Bednarz-Misa, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101321/
https://www.ncbi.nlm.nih.gov/pubmed/27339468
http://dx.doi.org/10.1007/s10616-016-9971-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101321/
https://www.ncbi.nlm.nih.gov/pubmed/27339468
http://dx.doi.org/10.1007/s10616-016-9971-4

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