Sequential and counter-selectable cassettes for fission yeast

BACKGROUND: Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of interest. In yeasts, this is achieved by PCR amplification of the cassette flank...

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Detalles Bibliográficos
Autores principales: Amelina, Hanna, Moiseeva, Vera, Collopy, Laura Catharine, Pearson, Siân Rosanna, Armstrong, Christine Anne, Tomita, Kazunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101803/
https://www.ncbi.nlm.nih.gov/pubmed/27825338
http://dx.doi.org/10.1186/s12896-016-0307-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101803/
https://www.ncbi.nlm.nih.gov/pubmed/27825338
http://dx.doi.org/10.1186/s12896-016-0307-4

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