A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection

We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of...

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Detalles Bibliográficos
Autores principales: Kashida, Hiromu, Morimoto, Kazuhiro, Asanuma, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Focus on Nanomedicine molecular science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101869/
https://www.ncbi.nlm.nih.gov/pubmed/27877879
http://dx.doi.org/10.1080/14686996.2016.1182412

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101869/
https://www.ncbi.nlm.nih.gov/pubmed/27877879
http://dx.doi.org/10.1080/14686996.2016.1182412

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