A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection

We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of...

Descripción completa

Detalles Bibliográficos
Autores principales: Kashida, Hiromu, Morimoto, Kazuhiro, Asanuma, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Focus on Nanomedicine molecular science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101869/
https://www.ncbi.nlm.nih.gov/pubmed/27877879
http://dx.doi.org/10.1080/14686996.2016.1182412
_version_ 1782466367095570432
author Kashida, Hiromu
Morimoto, Kazuhiro
Asanuma, Hiroyuki
author_facet Kashida, Hiromu
Morimoto, Kazuhiro
Asanuma, Hiroyuki
author_sort Kashida, Hiromu
collection PubMed
description We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of Cy3 was efficiently quenched. Hybridization with the target RNA disrupted this interaction and resulted in Cy3 emission. Under optimized conditions, the signal to background ratio was as high as 180. We demonstrated specific detection of target RNA in cells using a wash-free FISH protocol.
format Online
Article
Text
id pubmed-5101869
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-51018692016-11-22 A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection Kashida, Hiromu Morimoto, Kazuhiro Asanuma, Hiroyuki Sci Technol Adv Mater Focus on Nanomedicine molecular science We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of Cy3 was efficiently quenched. Hybridization with the target RNA disrupted this interaction and resulted in Cy3 emission. Under optimized conditions, the signal to background ratio was as high as 180. We demonstrated specific detection of target RNA in cells using a wash-free FISH protocol. Taylor & Francis 2016-07-05 /pmc/articles/PMC5101869/ /pubmed/27877879 http://dx.doi.org/10.1080/14686996.2016.1182412 Text en © 2016 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/)which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Focus on Nanomedicine molecular science
Kashida, Hiromu
Morimoto, Kazuhiro
Asanuma, Hiroyuki
A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title_full A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title_fullStr A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title_full_unstemmed A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title_short A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
title_sort stem-less probe using spontaneous pairing between cy3 and quencher for rna detection
topic Focus on Nanomedicine molecular science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101869/
https://www.ncbi.nlm.nih.gov/pubmed/27877879
http://dx.doi.org/10.1080/14686996.2016.1182412
work_keys_str_mv AT kashidahiromu astemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection
AT morimotokazuhiro astemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection
AT asanumahiroyuki astemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection
AT kashidahiromu stemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection
AT morimotokazuhiro stemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection
AT asanumahiroyuki stemlessprobeusingspontaneouspairingbetweency3andquencherforrnadetection

Ejemplares similares