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A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection
We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of...
Autores principales: | Kashida, Hiromu, Morimoto, Kazuhiro, Asanuma, Hiroyuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101869/ https://www.ncbi.nlm.nih.gov/pubmed/27877879 http://dx.doi.org/10.1080/14686996.2016.1182412 |
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