Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis

CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos. We show that incorporation of ribozymes increases the types of promoters and number of target sites availab...

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Detalles Bibliográficos
Autores principales: Lee, Raymond Teck Ho, Ng, Ashley Shu Mei, Ingham, Philip W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104441/
https://www.ncbi.nlm.nih.gov/pubmed/27832146
http://dx.doi.org/10.1371/journal.pone.0166020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104441/
https://www.ncbi.nlm.nih.gov/pubmed/27832146
http://dx.doi.org/10.1371/journal.pone.0166020

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