Cargando…

One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation

BACKGROUND: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Smith, Jordan L., Lee, Liam C., Read, Abigail, Li, Qiuning, Yu, Bing, Lee, Chih-Shia, Luo, Ji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106784/ https://www.ncbi.nlm.nih.gov/pubmed/27891214 http://dx.doi.org/10.1186/s13578-016-0122-6

Ejemplares similares

Development of immortalized mouse aortic endothelial cell lines
por: Ni, Chih-Wen, et al.
Publicado: (2014)

Immortalized cells and one oncogene in malignant transformation: old insights on new explanation
por: Kavsan, Vadym M, et al.
Publicado: (2011)

Visualisation of Multiple Tight Junctional Complexes in Human Airway Epithelial Cells
por: Buckley, Alysia G., et al.
Publicado: (2018)

Target Mechanisms of the Cyanotoxin Cylindrospermopsin in Immortalized Human Airway Epithelial Cells
por: Ziesemer, Sabine, et al.
Publicado: (2022)

Establishment of an immortalized human endometrial stromal cell line with functional responses to ovarian stimuli
por: Yuhki, Munehiro, et al.
Publicado: (2011)

Malignant transformation of immortalized human bronchial epithelial cells by asbestos fibers.
por: Hei, T K, et al.
Publicado: (1997)

One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation
por: Fan, Ying-lun, et al.
Publicado: (2020)

Neuromuscular electrical stimulation promotes development in mice of mature human muscle from immortalized human myoblasts
por: Sakellariou, Paraskevi, et al.
Publicado: (2016)

Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression
por: Gordon, Katrina, et al.
Publicado: (2014)

Loss of SELENOF Induces the Transformed Phenotype in Human Immortalized Prostate Epithelial Cells
por: Hong, Lenny K., et al.
Publicado: (2021)

Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells
por: Nakauchi, Mina, et al.
Publicado: (2019)

Cytogenetic characterization and H-ras associated transformation of immortalized human mammary epithelial cells
por: Rao, Krishna, et al.
Publicado: (2006)

Pyrosequencing™ : A one-step method for high resolution HLA typing
por: Ramon, Daniel, et al.
Publicado: (2003)

Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells
por: Geng, Fengxue, et al.
Publicado: (2017)

Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
por: Patel, Pranav, et al.
Publicado: (2013)

Capability of airline jets as an observation platform for noctilucent clouds at middle latitudes
por: Suzuki, Hidehiko, et al.
Publicado: (2022)

Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation
por: Weiand, Matthias, et al.
Publicado: (2022)

Cell-based assay for the detection of chemically induced cellular stress by immortalized untransformed transgenic hepatocytes
por: Sacco, Maria Grazia, et al.
Publicado: (2004)

Arsenic Compromises Conducting Airway Epithelial Barrier Properties in Primary Mouse and Immortalized Human Cell Cultures
por: Sherwood, Cara L., et al.
Publicado: (2013)

High-throughput malaria serosurveillance using a one-step multiplex bead assay
por: Rogier, Eric, et al.
Publicado: (2019)

Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe
por: Hong, JiYoung, et al.
Publicado: (2011)

Development and validation of an HILIC–MS/MS method by one-step precipitation for chloroquine in miniature pig plasma
por: Wang, Zhan-Zhang, et al.
Publicado: (2016)

Establishment of microbial model communities capable of removing trace organic chemicals for biotransformation mechanisms research
por: Cao, Lijia, et al.
Publicado: (2023)

Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell
por: Xie, Lingxia, et al.
Publicado: (2014)

Coevolution of neoplastic epithelial cells and multilineage stroma via polyploid giant cells during immortalization and transformation of mullerian epithelial cells
por: Zhang, Shiwu, et al.
Publicado: (2016)

Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses
por: Saxena, Parag, et al.
Publicado: (2008)

One-step concentration of malarial parasite-infected red blood cells and removal of contaminating white blood cells
por: Trang, Dai Thi Xuan, et al.
Publicado: (2004)

One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas
por: Croner, Roland S, et al.
Publicado: (2010)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Rapid Detection of high-level oncogene amplifications in ultrasonic surgical aspirations of brain tumors
por: Truong, Long N, et al.
Publicado: (2012)

One-step detection of Bean pod mottle virus in soybean seeds by the reverse-transcription loop-mediated isothermal amplification
por: Wei, Qi-Wei, et al.
Publicado: (2012)

Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana
por: Davis, Amanda M, et al.
Publicado: (2009)

Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation
por: Spadafora, Carmenza, et al.
Publicado: (2011)

Development of an innovative and sustainable one-step method for rapid plant DNA isolation for targeted PCR using magnetic ionic liquids
por: Marengo, Arianna, et al.
Publicado: (2019)

Systematic assessment of secondary bile acid metabolism in gut microbes reveals distinct metabolic capabilities in inflammatory bowel disease
por: Heinken, Almut, et al.
Publicado: (2019)

A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation
por: Harrison, Samuel J, et al.
Publicado: (2006)

One-pot and one-step automated radio-synthesis of [(18)F]AlF-FAPI-74 using a multi purpose synthesizer: a proof-of-concept experiment
por: Naka, Sadahiro, et al.
Publicado: (2021)

A novel MCF-10A line allowing conditional oncogene expression in 3D culture
por: Herr, Ricarda, et al.
Publicado: (2011)

A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence
por: Komseli, Eirini-Stavroula, et al.
Publicado: (2018)

Simple steps to develop trial follow-up procedures
por: McCarthy, Ona, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_5v0vggm90b8tlee39v83tnron1