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CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening

BACKGROUND: The bacterial CRISPR system is fast becoming the most popular genetic and epigenetic engineering tool due to its universal applicability and adaptability. The desire to deploy CRISPR-based methods in a large variety of species and contexts has created an urgent need for the development o...

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Detalles Bibliográficos
Autores principales: Köferle, Anna, Worf, Karolina, Breunig, Christopher, Baumann, Valentin, Herrero, Javier, Wiesbeck, Maximilian, Hutter, Lukas H., Götz, Magdalena, Fuchs, Christiane, Beck, Stephan, Stricker, Stefan H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109649/
https://www.ncbi.nlm.nih.gov/pubmed/27842490
http://dx.doi.org/10.1186/s12864-016-3268-z