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Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
BACKGROUND: Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compa...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109708/ https://www.ncbi.nlm.nih.gov/pubmed/27842593 http://dx.doi.org/10.1186/s12934-016-0594-4 |