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Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit

Induction of hyperbilirubinemia in experimental rabbits by phenylhydrazine was optimized in terms of dose, dose interval and number of doses using response surface methodology. Central Composite Design was employed using five levels for each of the three input variables. Degree of hyperbilirubinemia...

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Detalles Bibliográficos
Autores principales: Nawaz, Haq, Shad, Muhammad Aslam, Iqbal, Mohammad Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111839/
https://www.ncbi.nlm.nih.gov/pubmed/27210076
http://dx.doi.org/10.1538/expanim.16-0011
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author Nawaz, Haq
Shad, Muhammad Aslam
Iqbal, Mohammad Saeed
author_facet Nawaz, Haq
Shad, Muhammad Aslam
Iqbal, Mohammad Saeed
author_sort Nawaz, Haq
collection PubMed
description Induction of hyperbilirubinemia in experimental rabbits by phenylhydrazine was optimized in terms of dose, dose interval and number of doses using response surface methodology. Central Composite Design was employed using five levels for each of the three input variables. Degree of hyperbilirubinemia was measured in terms of bilirubin level in serum of animals. A dose dependent significant elevation (P<0.05) of total serum bilirubin level was observed which was optimized by using eight factorial, six axial and six central points as suggested by experimental design. Optimum levels of phenylhydrazine dose, total number of doses and a dose interval to achieve maximum elevation (4.06 mg/dl(−1)) of total serum bilirubin were found to be 11.56 mg/kg(−1) body weight, 8 and 24.65 h, respectively. The induction procedure was validated by performing five replicate experiments on a group of five animals which showed 3.56 ± 0.47 mg/kg(−1) body weight elevation in total serum bilirubin level.
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spelling pubmed-51118392016-11-17 Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit Nawaz, Haq Shad, Muhammad Aslam Iqbal, Mohammad Saeed Exp Anim Original Induction of hyperbilirubinemia in experimental rabbits by phenylhydrazine was optimized in terms of dose, dose interval and number of doses using response surface methodology. Central Composite Design was employed using five levels for each of the three input variables. Degree of hyperbilirubinemia was measured in terms of bilirubin level in serum of animals. A dose dependent significant elevation (P<0.05) of total serum bilirubin level was observed which was optimized by using eight factorial, six axial and six central points as suggested by experimental design. Optimum levels of phenylhydrazine dose, total number of doses and a dose interval to achieve maximum elevation (4.06 mg/dl(−1)) of total serum bilirubin were found to be 11.56 mg/kg(−1) body weight, 8 and 24.65 h, respectively. The induction procedure was validated by performing five replicate experiments on a group of five animals which showed 3.56 ± 0.47 mg/kg(−1) body weight elevation in total serum bilirubin level. Japanese Association for Laboratory Animal Science 2016-05-20 2016 /pmc/articles/PMC5111839/ /pubmed/27210076 http://dx.doi.org/10.1538/expanim.16-0011 Text en ©2016 Japanese Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original
Nawaz, Haq
Shad, Muhammad Aslam
Iqbal, Mohammad Saeed
Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title_full Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title_fullStr Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title_full_unstemmed Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title_short Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
title_sort optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111839/
https://www.ncbi.nlm.nih.gov/pubmed/27210076
http://dx.doi.org/10.1538/expanim.16-0011
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