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Bright blue-shifted fluorescent proteins with Cys in the GAF domain engineered from bacterial phytochromes: fluorescence mechanisms and excited-state dynamics

Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are of great interest for in vivo imaging. They utilize biliverdin (BV) as a chromophore, which is a heme degradation product, and therefore they are straightforward to use in mammalian tissues. Here, we repo...

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Detalles Bibliográficos
Autores principales: Hontani, Yusaku, Shcherbakova, Daria M., Baloban, Mikhail, Zhu, Jingyi, Verkhusha, Vladislav V., Kennis, John T. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114657/
https://www.ncbi.nlm.nih.gov/pubmed/27857208
http://dx.doi.org/10.1038/srep37362