In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells

Firefly luciferase (Fluc) is frequently used to report circadian gene expression rhythms in mammalian cells and tissues. During longitudinal assays it is generally assumed that enzymatic substrates are in saturating excess, such that total bioluminescence is directly proportional to Fluc protein lev...

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Detalles Bibliográficos
Autores principales: Feeney, Kevin A., Putker, Marrit, Brancaccio, Marco, O’Neill, John S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2016
Materias:
Technique
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117186/
https://www.ncbi.nlm.nih.gov/pubmed/28112045
http://dx.doi.org/10.1177/0748730416668898

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117186/
https://www.ncbi.nlm.nih.gov/pubmed/28112045
http://dx.doi.org/10.1177/0748730416668898

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