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In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells
Firefly luciferase (Fluc) is frequently used to report circadian gene expression rhythms in mammalian cells and tissues. During longitudinal assays it is generally assumed that enzymatic substrates are in saturating excess, such that total bioluminescence is directly proportional to Fluc protein lev...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
SAGE Publications
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117186/ https://www.ncbi.nlm.nih.gov/pubmed/28112045 http://dx.doi.org/10.1177/0748730416668898 |