Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis

Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classifi...

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Autores principales: Jung, Sung-Min, Hur, Youn-Young, Preece, John E., Fiehn, Oliver, Kim, Young-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117857/
https://www.ncbi.nlm.nih.gov/pubmed/27904455
http://dx.doi.org/10.5423/PPJ.FT.08.2016.0163
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author Jung, Sung-Min
Hur, Youn-Young
Preece, John E.
Fiehn, Oliver
Kim, Young-Ho
author_facet Jung, Sung-Min
Hur, Youn-Young
Preece, John E.
Fiehn, Oliver
Kim, Young-Ho
author_sort Jung, Sung-Min
collection PubMed
description Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses, respectively. In this, 4, 4, and 2 Vitis species were classified into RR, SR, and SS, respectively. Gas chromatography mass spectrometry (GC-MS) analysis of the grapevine stem metabolites with A. vitis infection showed 134 metabolites in various compound classes critically occurred, which were differentially clustered with the response types by the principal component analysis. Multivariate analysis of the metabolite profile revealed that 11 metabolites increased significantly in relation to the response types, mostly at post-inoculation stages, more prevalently (8 metabolites) at two days after inoculation than other stages, and more related to SS (7 metabolites) than RR (3 metabolites) or SR (one metabolite). This suggests most of the disease-related metabolites may be rarely pre-existing but mostly induced by pathogen infection largely for facilitating gall development except stilbene compound resveratrol, a phytoalexin that may be involved in the resistance response. All of these aspects may be used for the selection of resistant grapevine cultivars and their rootstocks for the control of the crown gall disease of the grapevine.
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spelling pubmed-51178572016-12-01 Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis Jung, Sung-Min Hur, Youn-Young Preece, John E. Fiehn, Oliver Kim, Young-Ho Plant Pathol J Research Article Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses, respectively. In this, 4, 4, and 2 Vitis species were classified into RR, SR, and SS, respectively. Gas chromatography mass spectrometry (GC-MS) analysis of the grapevine stem metabolites with A. vitis infection showed 134 metabolites in various compound classes critically occurred, which were differentially clustered with the response types by the principal component analysis. Multivariate analysis of the metabolite profile revealed that 11 metabolites increased significantly in relation to the response types, mostly at post-inoculation stages, more prevalently (8 metabolites) at two days after inoculation than other stages, and more related to SS (7 metabolites) than RR (3 metabolites) or SR (one metabolite). This suggests most of the disease-related metabolites may be rarely pre-existing but mostly induced by pathogen infection largely for facilitating gall development except stilbene compound resveratrol, a phytoalexin that may be involved in the resistance response. All of these aspects may be used for the selection of resistant grapevine cultivars and their rootstocks for the control of the crown gall disease of the grapevine. Korean Society of Plant Pathology 2016-12 2016-12-01 /pmc/articles/PMC5117857/ /pubmed/27904455 http://dx.doi.org/10.5423/PPJ.FT.08.2016.0163 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jung, Sung-Min
Hur, Youn-Young
Preece, John E.
Fiehn, Oliver
Kim, Young-Ho
Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title_full Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title_fullStr Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title_full_unstemmed Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title_short Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
title_sort profiling of disease-related metabolites in grapevine internode tissues infected with agrobacterium vitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117857/
https://www.ncbi.nlm.nih.gov/pubmed/27904455
http://dx.doi.org/10.5423/PPJ.FT.08.2016.0163
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