Cas9-catalyzed DNA Cleavage Generates Staggered Ends: Evidence from Molecular Dynamics Simulations

The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (spCas9) along with a single guide RNA (sgRNA) has emerged as a versatile toolbox for genome editing. Despite recent advances in the mechanism studies on spCas9-sgRNA-mediated double-stranded DNA (dsDNA) recognition and cleavage, it...

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Detalles Bibliográficos
Autores principales: Zuo, Zhicheng, Liu, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118739/
https://www.ncbi.nlm.nih.gov/pubmed/27874072
http://dx.doi.org/10.1038/srep37584

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118739/
https://www.ncbi.nlm.nih.gov/pubmed/27874072
http://dx.doi.org/10.1038/srep37584

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