A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA...

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Detalles Bibliográficos
Autores principales: Su, Tianyuan, Liu, Fapeng, Gu, Pengfei, Jin, Haiying, Chang, Yizhao, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121644/
https://www.ncbi.nlm.nih.gov/pubmed/27883076
http://dx.doi.org/10.1038/srep37895

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121644/
https://www.ncbi.nlm.nih.gov/pubmed/27883076
http://dx.doi.org/10.1038/srep37895

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