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A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA...
Autores principales: | Su, Tianyuan, Liu, Fapeng, Gu, Pengfei, Jin, Haiying, Chang, Yizhao, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121644/ https://www.ncbi.nlm.nih.gov/pubmed/27883076 http://dx.doi.org/10.1038/srep37895 |
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