Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

BACKGROUND: CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of Thalassiosira pseudonana, a mode...

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Detalles Bibliográficos
Autores principales: Hopes, Amanda, Nekrasov, Vladimir, Kamoun, Sophien, Mock, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121945/
https://www.ncbi.nlm.nih.gov/pubmed/27904648
http://dx.doi.org/10.1186/s13007-016-0148-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121945/
https://www.ncbi.nlm.nih.gov/pubmed/27904648
http://dx.doi.org/10.1186/s13007-016-0148-0

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