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Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
BACKGROUND: CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of Thalassiosira pseudonana, a mode...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5121945/ https://www.ncbi.nlm.nih.gov/pubmed/27904648 http://dx.doi.org/10.1186/s13007-016-0148-0 |