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Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy

BACKGROUND: Changes in immunoglobulin G (IgG) constant domain (Fc) glycosylation are associated with changes in rheumatoid arthritis (RA) disease activity in response to pregnancy. Here, we sought to determine whether the same holds true for variable domain (Fab) glycosylation. METHODS: IgGs were ca...

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Autores principales: Bondt, Albert, Wuhrer, Manfred, Kuijper, T. Martijn, Hazes, Johanna M. W., Dolhain, Radboud J. E. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123206/
https://www.ncbi.nlm.nih.gov/pubmed/27887659
http://dx.doi.org/10.1186/s13075-016-1172-1
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author Bondt, Albert
Wuhrer, Manfred
Kuijper, T. Martijn
Hazes, Johanna M. W.
Dolhain, Radboud J. E. M.
author_facet Bondt, Albert
Wuhrer, Manfred
Kuijper, T. Martijn
Hazes, Johanna M. W.
Dolhain, Radboud J. E. M.
author_sort Bondt, Albert
collection PubMed
description BACKGROUND: Changes in immunoglobulin G (IgG) constant domain (Fc) glycosylation are associated with changes in rheumatoid arthritis (RA) disease activity in response to pregnancy. Here, we sought to determine whether the same holds true for variable domain (Fab) glycosylation. METHODS: IgGs were captured from RA and control sera obtained before (RA only), during and after pregnancy, followed by Fc and Fab separation, glycan release, and mass spectrometric detection. In parallel, glycans from intact IgG were analysed. The data was used to calculate glycosylation traits, and to estimate the level of Fab glycosylation. RESULTS: The overall level of Fab glycosylation was increased in RA patients compared to controls, while no differences in Fab glycosylation patterns were found. For the Fc and intact IgG (Total) previously observed differences in galactosylation and bisection were confirmed. Furthermore, increased galactosylation of Fc and Total were associated with lower disease activity and autoantibody positivity. In addition, the change in Fc galactosylation associated with the change in disease activity during pregnancy and after delivery, while this was not the case for Fab. CONCLUSIONS: In contrast to changes in Fc glycosylation, changes in Fab glycosylation are not associated with improvement of RA during pregnancy and arthritis flare after delivery. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-016-1172-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-51232062016-12-06 Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy Bondt, Albert Wuhrer, Manfred Kuijper, T. Martijn Hazes, Johanna M. W. Dolhain, Radboud J. E. M. Arthritis Res Ther Research Article BACKGROUND: Changes in immunoglobulin G (IgG) constant domain (Fc) glycosylation are associated with changes in rheumatoid arthritis (RA) disease activity in response to pregnancy. Here, we sought to determine whether the same holds true for variable domain (Fab) glycosylation. METHODS: IgGs were captured from RA and control sera obtained before (RA only), during and after pregnancy, followed by Fc and Fab separation, glycan release, and mass spectrometric detection. In parallel, glycans from intact IgG were analysed. The data was used to calculate glycosylation traits, and to estimate the level of Fab glycosylation. RESULTS: The overall level of Fab glycosylation was increased in RA patients compared to controls, while no differences in Fab glycosylation patterns were found. For the Fc and intact IgG (Total) previously observed differences in galactosylation and bisection were confirmed. Furthermore, increased galactosylation of Fc and Total were associated with lower disease activity and autoantibody positivity. In addition, the change in Fc galactosylation associated with the change in disease activity during pregnancy and after delivery, while this was not the case for Fab. CONCLUSIONS: In contrast to changes in Fc glycosylation, changes in Fab glycosylation are not associated with improvement of RA during pregnancy and arthritis flare after delivery. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-016-1172-1) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-25 2016 /pmc/articles/PMC5123206/ /pubmed/27887659 http://dx.doi.org/10.1186/s13075-016-1172-1 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bondt, Albert
Wuhrer, Manfred
Kuijper, T. Martijn
Hazes, Johanna M. W.
Dolhain, Radboud J. E. M.
Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title_full Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title_fullStr Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title_full_unstemmed Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title_short Fab glycosylation of immunoglobulin G does not associate with improvement of rheumatoid arthritis during pregnancy
title_sort fab glycosylation of immunoglobulin g does not associate with improvement of rheumatoid arthritis during pregnancy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123206/
https://www.ncbi.nlm.nih.gov/pubmed/27887659
http://dx.doi.org/10.1186/s13075-016-1172-1
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