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Traditional versus 3′ RNA-seq in a non-model species
One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extra...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124356/ https://www.ncbi.nlm.nih.gov/pubmed/27909684 http://dx.doi.org/10.1016/j.gdata.2016.11.002 |