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Traditional versus 3′ RNA-seq in a non-model species

One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extra...

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Detalles Bibliográficos
Autores principales: Tandonnet, Sophie, Torres, Tatiana Teixeira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124356/
https://www.ncbi.nlm.nih.gov/pubmed/27909684
http://dx.doi.org/10.1016/j.gdata.2016.11.002