Internal epitope tagging informed by relative lack of sequence conservation

Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by inte...

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Detalles Bibliográficos
Autores principales: Burg, Leonard, Zhang, Karen, Bonawitz, Tristan, Grajevskaja, Viktorija, Bellipanni, Gianfranco, Waring, Richard, Balciunas, Darius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125009/
https://www.ncbi.nlm.nih.gov/pubmed/27892520
http://dx.doi.org/10.1038/srep36986

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125009/
https://www.ncbi.nlm.nih.gov/pubmed/27892520
http://dx.doi.org/10.1038/srep36986

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