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Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats

Escherichia coli are important indicator organisms, used routinely for the monitoring of water and food safety. For quick, sensitive and real-time detection of E. coli we developed a 2′F modified RNA aptamer Ec3, by Cell-SELEX. The 31 nucleotide truncated Ec3 demonstrated improved binding and low na...

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Detalles Bibliográficos
Autores principales: Dua, Pooja, Ren, Shuo, Lee, Sang Wook, Kim, Joon-Ki, Shin, Hye-su, Jeong, OK-Chan, Kim, Soyoun, Lee, Dong-Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125936/
https://www.ncbi.nlm.nih.gov/pubmed/27871171
http://dx.doi.org/10.14348/molcells.2016.0167