Normalization with Corresponding Naïve Tissue Minimizes Bias Caused by Commercial Reverse Transcription Kits on Quantitative Real-Time PCR Results

Real-time reverse transcription polymerase chain reaction (PCR) is the gold standard for expression analysis. Designed to improve reproducibility and sensitivity, commercial kits are commonly used for the critical step of cDNA synthesis. The present study was designed to determine the impact of thes...

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Detalles Bibliográficos
Autores principales: Garcia-Bardon, Andreas, Thal, Serge C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127578/
https://www.ncbi.nlm.nih.gov/pubmed/27898720
http://dx.doi.org/10.1371/journal.pone.0167209

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127578/
https://www.ncbi.nlm.nih.gov/pubmed/27898720
http://dx.doi.org/10.1371/journal.pone.0167209

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