Efficient generation of selection‐gene‐free rat knockout models by homologous recombination in ES cells

Embryonic stem cell (ES cell)‐based rat knockout technology, although successfully developed in 2010, has seen very limited usage to date due to low targeting efficiency and a lack of optimized procedures. In this study, we performed gene targeting in ES cells from the Sprague–Dawley (SD) and the Fi...

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Detalles Bibliográficos
Autores principales: Lan, He, Li, Shuping, Guo, Zihang, Men, Hongsheng, Wu, Yuanyuan, Li, Ning, Bryda, Elizabeth C., Capecchi, Mario R., Wu, Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129459/
https://www.ncbi.nlm.nih.gov/pubmed/27597178
http://dx.doi.org/10.1002/1873-3468.12388

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129459/
https://www.ncbi.nlm.nih.gov/pubmed/27597178
http://dx.doi.org/10.1002/1873-3468.12388

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