Cargando…

Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5

Assembly of the spliceosomal small nuclear ribonucleoparticle (snRNP) core requires the participation of the multisubunit SMN (survival of motor neuron) complex, which contains SMN and several Gemin proteins. The SMN and Gemin2 subunits directly bind Sm proteins, and Gemin5 is required for snRNP bio...

Descripción completa

Detalles Bibliográficos
Autores principales: Jin, Wenxing, Wang, Yi, Liu, Chao-Pei, Yang, Na, Jin, Mingliang, Cong, Yao, Wang, Mingzhu, Xu, Rui-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131779/
https://www.ncbi.nlm.nih.gov/pubmed/27881601
http://dx.doi.org/10.1101/gad.291377.116
_version_ 1782470946989277184
author Jin, Wenxing
Wang, Yi
Liu, Chao-Pei
Yang, Na
Jin, Mingliang
Cong, Yao
Wang, Mingzhu
Xu, Rui-Ming
author_facet Jin, Wenxing
Wang, Yi
Liu, Chao-Pei
Yang, Na
Jin, Mingliang
Cong, Yao
Wang, Mingzhu
Xu, Rui-Ming
author_sort Jin, Wenxing
collection PubMed
description Assembly of the spliceosomal small nuclear ribonucleoparticle (snRNP) core requires the participation of the multisubunit SMN (survival of motor neuron) complex, which contains SMN and several Gemin proteins. The SMN and Gemin2 subunits directly bind Sm proteins, and Gemin5 is required for snRNP biogenesis and has been implicated in snRNA recognition. The RNA sequence required for snRNP assembly includes the Sm site and an adjacent 3′ stem–loop, but a precise understanding of Gemin5's RNA-binding specificity is lacking. Here we show that the N-terminal half of Gemin5, which is composed of two juxtaposed seven-bladed WD40 repeat domains, recognizes the Sm site. The tandem WD40 repeat domains are rigidly held together to form a contiguous RNA-binding surface. RNA-contacting residues are located mostly on loops between β strands on the apical surface of the WD40 domains. Structural and biochemical analyses show that base-stacking interactions involving four aromatic residues and hydrogen bonding by a pair of arginines are crucial for specific recognition of the Sm sequence. We also show that an adenine immediately 5′ to the Sm site is required for efficient binding and that Gemin5 can bind short RNA oligos in an alternative mode. Our results provide mechanistic understandings of Gemin5's snRNA-binding specificity as well as valuable insights into the molecular mechanism of RNA binding by WD40 repeat proteins in general.
format Online
Article
Text
id pubmed-5131779
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-51317792017-05-01 Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5 Jin, Wenxing Wang, Yi Liu, Chao-Pei Yang, Na Jin, Mingliang Cong, Yao Wang, Mingzhu Xu, Rui-Ming Genes Dev Research Paper Assembly of the spliceosomal small nuclear ribonucleoparticle (snRNP) core requires the participation of the multisubunit SMN (survival of motor neuron) complex, which contains SMN and several Gemin proteins. The SMN and Gemin2 subunits directly bind Sm proteins, and Gemin5 is required for snRNP biogenesis and has been implicated in snRNA recognition. The RNA sequence required for snRNP assembly includes the Sm site and an adjacent 3′ stem–loop, but a precise understanding of Gemin5's RNA-binding specificity is lacking. Here we show that the N-terminal half of Gemin5, which is composed of two juxtaposed seven-bladed WD40 repeat domains, recognizes the Sm site. The tandem WD40 repeat domains are rigidly held together to form a contiguous RNA-binding surface. RNA-contacting residues are located mostly on loops between β strands on the apical surface of the WD40 domains. Structural and biochemical analyses show that base-stacking interactions involving four aromatic residues and hydrogen bonding by a pair of arginines are crucial for specific recognition of the Sm sequence. We also show that an adenine immediately 5′ to the Sm site is required for efficient binding and that Gemin5 can bind short RNA oligos in an alternative mode. Our results provide mechanistic understandings of Gemin5's snRNA-binding specificity as well as valuable insights into the molecular mechanism of RNA binding by WD40 repeat proteins in general. Cold Spring Harbor Laboratory Press 2016-11-01 /pmc/articles/PMC5131779/ /pubmed/27881601 http://dx.doi.org/10.1101/gad.291377.116 Text en © 2016 Jin et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Paper
Jin, Wenxing
Wang, Yi
Liu, Chao-Pei
Yang, Na
Jin, Mingliang
Cong, Yao
Wang, Mingzhu
Xu, Rui-Ming
Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title_full Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title_fullStr Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title_full_unstemmed Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title_short Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5
title_sort structural basis for snrna recognition by the double-wd40 repeat domain of gemin5
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131779/
https://www.ncbi.nlm.nih.gov/pubmed/27881601
http://dx.doi.org/10.1101/gad.291377.116
work_keys_str_mv AT jinwenxing structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT wangyi structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT liuchaopei structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT yangna structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT jinmingliang structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT congyao structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT wangmingzhu structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5
AT xuruiming structuralbasisforsnrnarecognitionbythedoublewd40repeatdomainofgemin5