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Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors

OBJECTIVE: We have previously shown alterations in the composition of the gut microbiota in children with enthesitis-related arthritis (ERA). To explore the mechanisms by which an altered microbiota might predispose to arthritis, we performed metabolomic profiling of fecal samples of children with E...

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Autores principales: Stoll, Matthew L, Kumar, Ranjit, Lefkowitz, Elliot, Cron, Randy Q, Morrow, Casey D, Barnes, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133160/
https://www.ncbi.nlm.nih.gov/pubmed/27786174
http://dx.doi.org/10.1038/gene.2016.38
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author Stoll, Matthew L
Kumar, Ranjit
Lefkowitz, Elliot
Cron, Randy Q
Morrow, Casey D
Barnes, Stephen
author_facet Stoll, Matthew L
Kumar, Ranjit
Lefkowitz, Elliot
Cron, Randy Q
Morrow, Casey D
Barnes, Stephen
author_sort Stoll, Matthew L
collection PubMed
description OBJECTIVE: We have previously shown alterations in the composition of the gut microbiota in children with enthesitis-related arthritis (ERA). To explore the mechanisms by which an altered microbiota might predispose to arthritis, we performed metabolomic profiling of fecal samples of children with ERA. METHODS: Fecal samples were collected from two cohorts of children with ERA and healthy control subjects. Nano-liquid chromatography – mass spectroscopy (LC-MS) was performed on the fecal water homogenates with identification based upon mass: charge ratios. Sequencing of the 16S ribosomal DNA (rDNA) on the same stool specimens was performed. RESULTS: In both sets of subjects, patients demonstrated lower diversity of ions and under-representation of multiple metabolic pathways, including the tryptophan metabolism pathway. For example, in the first cohort, out of 1,500 negatively charged ions, 154 were lower in ERA patients, compared to only one that was higher. Imputed functional annotation of the 16S rDNA sequence data demonstrated significantly fewer microbial genes associated with metabolic processes in the patients compared to the controls (77 million versus 58 million, p = 0.050.) CONCLUSIONS: Diminished metabolic diversity and alterations in the tryptophan metabolism pathway may be a feature of ERA.
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spelling pubmed-51331602017-04-27 Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors Stoll, Matthew L Kumar, Ranjit Lefkowitz, Elliot Cron, Randy Q Morrow, Casey D Barnes, Stephen Genes Immun Article OBJECTIVE: We have previously shown alterations in the composition of the gut microbiota in children with enthesitis-related arthritis (ERA). To explore the mechanisms by which an altered microbiota might predispose to arthritis, we performed metabolomic profiling of fecal samples of children with ERA. METHODS: Fecal samples were collected from two cohorts of children with ERA and healthy control subjects. Nano-liquid chromatography – mass spectroscopy (LC-MS) was performed on the fecal water homogenates with identification based upon mass: charge ratios. Sequencing of the 16S ribosomal DNA (rDNA) on the same stool specimens was performed. RESULTS: In both sets of subjects, patients demonstrated lower diversity of ions and under-representation of multiple metabolic pathways, including the tryptophan metabolism pathway. For example, in the first cohort, out of 1,500 negatively charged ions, 154 were lower in ERA patients, compared to only one that was higher. Imputed functional annotation of the 16S rDNA sequence data demonstrated significantly fewer microbial genes associated with metabolic processes in the patients compared to the controls (77 million versus 58 million, p = 0.050.) CONCLUSIONS: Diminished metabolic diversity and alterations in the tryptophan metabolism pathway may be a feature of ERA. 2016-10-27 2016-12 /pmc/articles/PMC5133160/ /pubmed/27786174 http://dx.doi.org/10.1038/gene.2016.38 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Stoll, Matthew L
Kumar, Ranjit
Lefkowitz, Elliot
Cron, Randy Q
Morrow, Casey D
Barnes, Stephen
Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title_full Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title_fullStr Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title_full_unstemmed Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title_short Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
title_sort fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133160/
https://www.ncbi.nlm.nih.gov/pubmed/27786174
http://dx.doi.org/10.1038/gene.2016.38
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