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Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

BACKGROUND: Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escherichia coli Rosetta (DE3) in the form of inclusion bodies, which were refolded...

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Detalles Bibliográficos
Autores principales: Wang, Nan, Ren, Kai, Jia, Rong, Chen, Wenting, Sun, Ruirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134096/
https://www.ncbi.nlm.nih.gov/pubmed/27908283
http://dx.doi.org/10.1186/s12896-016-0317-2