Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells

The ability to produce extremely small and circular supercoiled vectors has opened new territory for improving non-viral gene therapy vectors. In this work, we compared transfection of supercoiled DNA vectors ranging from 383 to 4,548 bp, each encoding shRNA against GFP under control of the H1 promo...

Descripción completa

Detalles Bibliográficos
Autores principales: Hornstein, Benjamin D., Roman, Dany, Arévalo-Soliz, Lirio M., Engevik, Melinda A., Zechiedrich, Lynn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137892/
https://www.ncbi.nlm.nih.gov/pubmed/27918590
http://dx.doi.org/10.1371/journal.pone.0167537

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137892/
https://www.ncbi.nlm.nih.gov/pubmed/27918590
http://dx.doi.org/10.1371/journal.pone.0167537

Ejemplares similares