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Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells
The ability to produce extremely small and circular supercoiled vectors has opened new territory for improving non-viral gene therapy vectors. In this work, we compared transfection of supercoiled DNA vectors ranging from 383 to 4,548 bp, each encoding shRNA against GFP under control of the H1 promo...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137892/ https://www.ncbi.nlm.nih.gov/pubmed/27918590 http://dx.doi.org/10.1371/journal.pone.0167537 |