LOVTRAP, An Optogenetic System for Photo-induced Protein Dissociation

Here we introduce LOVTRAP, an optogenetic approach for reversible, light-induced protein dissociation. LOVTRAP is based on protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in K(d). By reversibly sequestering proteins at mitochondria, w...

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Detalles Bibliográficos
Autores principales: Wang, Hui, Vilela, Marco, Winkler, Andreas, Tarnawski, Miroslaw, Schlichting, Ilme, Yumerefendi, Hayretin, Kuhlman, Brian, Liu, Rihe, Danuser, Gaudenz, Hahn, Klaus M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137947/
https://www.ncbi.nlm.nih.gov/pubmed/27427858
http://dx.doi.org/10.1038/nmeth.3926

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137947/
https://www.ncbi.nlm.nih.gov/pubmed/27427858
http://dx.doi.org/10.1038/nmeth.3926

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