Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis

The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and re...

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Detalles Bibliográficos
Autores principales: Hussain, Hasnain, Chong, Nikson Fatt-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138438/
https://www.ncbi.nlm.nih.gov/pubmed/27995143
http://dx.doi.org/10.1155/2016/8041532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138438/
https://www.ncbi.nlm.nih.gov/pubmed/27995143
http://dx.doi.org/10.1155/2016/8041532

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