High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells i...

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Autores principales: Wevers, Nienke R., van Vught, Remko, Wilschut, Karlijn J., Nicolas, Arnaud, Chiang, Chiwan, Lanz, Henriette L., Trietsch, Sebastiaan J., Joore, Jos, Vulto, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146966/
https://www.ncbi.nlm.nih.gov/pubmed/27934939
http://dx.doi.org/10.1038/srep38856
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author Wevers, Nienke R.
van Vught, Remko
Wilschut, Karlijn J.
Nicolas, Arnaud
Chiang, Chiwan
Lanz, Henriette L.
Trietsch, Sebastiaan J.
Joore, Jos
Vulto, Paul
author_facet Wevers, Nienke R.
van Vught, Remko
Wilschut, Karlijn J.
Nicolas, Arnaud
Chiang, Chiwan
Lanz, Henriette L.
Trietsch, Sebastiaan J.
Joore, Jos
Vulto, Paul
author_sort Wevers, Nienke R.
collection PubMed
description With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells in a microfluidic platform. The high-throughput nature of the platform in combination with its compatibility with all standard laboratory equipment allows for parallel evaluation of compound effects.
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spelling pubmed-51469662016-12-16 High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform Wevers, Nienke R. van Vught, Remko Wilschut, Karlijn J. Nicolas, Arnaud Chiang, Chiwan Lanz, Henriette L. Trietsch, Sebastiaan J. Joore, Jos Vulto, Paul Sci Rep Article With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells in a microfluidic platform. The high-throughput nature of the platform in combination with its compatibility with all standard laboratory equipment allows for parallel evaluation of compound effects. Nature Publishing Group 2016-12-09 /pmc/articles/PMC5146966/ /pubmed/27934939 http://dx.doi.org/10.1038/srep38856 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wevers, Nienke R.
van Vught, Remko
Wilschut, Karlijn J.
Nicolas, Arnaud
Chiang, Chiwan
Lanz, Henriette L.
Trietsch, Sebastiaan J.
Joore, Jos
Vulto, Paul
High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title_full High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title_fullStr High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title_full_unstemmed High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title_short High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
title_sort high-throughput compound evaluation on 3d networks of neurons and glia in a microfluidic platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146966/
https://www.ncbi.nlm.nih.gov/pubmed/27934939
http://dx.doi.org/10.1038/srep38856
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