Labeling proteins inside living cells using external fluorophores for microscopy

Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG’s to small ligands, with high effi...

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Detalles Bibliográficos
Autores principales: Teng, Kai Wen, Ishitsuka, Yuji, Ren, Pin, Youn, Yeoan, Deng, Xiang, Ge, Pinghua, Lee, Sang Hak, Belmont, Andrew S, Selvin, Paul R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/
https://www.ncbi.nlm.nih.gov/pubmed/27935478
http://dx.doi.org/10.7554/eLife.20378

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/
https://www.ncbi.nlm.nih.gov/pubmed/27935478
http://dx.doi.org/10.7554/eLife.20378

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